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T. A. Bogush, S. A. Kaliuzhny, A. A. Basharina, E. A. Bogush, V. Yu. Kirsanov, V. S. Kosorukov, O. S. Burova, M. M. Davydov, M. A. Baryshnikova
Coexpression of vimentin and cytokeratins
in human melanoma cells lines
Abstract
Molecular
heterogeneity in 7 human metastatic melanoma cells lines was revealed by dual
immunofluorescent assay and flow cytometry. It has been evaluated that quite a
small population of cells (up to 10%) expressed only vimentin, the main marker
of melanoma. Only epithelial cytokeratins expression was revealed in more
significant amount of cells (from 25% to 50%). And finally, coexpression of vimentin
and cytokeratins was newly identified and dominant molecular phenotype (50–80%
cells expressed both markers). The authors expect that the letter phenotype of
melanoma cells may be a molecular marker of tumor aggressivenessand metastatic potential.
Key words: immunofluorescent assay, flow cytometry, vimentin,
cytokeratins, melanoma.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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