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V. D. Telitsin1, M. V. Semenova2*, A. V. Gusakov1, A. P. Sinitsyn1, 2
Using 2,6-dimethoxyphenol for
determination of polysaccharide monooxygenase content in multienzyme coctails
Abstract
Express method of quantitative
determination of lytic polysaccharide monooxygenase (LPMO) content in the
multienzyme preparations produced by mutant strains of filamentous fungi was
developed. The method is based on using 2,6-dimethoxyphenol and hydrogen
peroxide as cosubstrates for a non-specific reaction catalyzed by LPMO, leading
to the formation of a chromogenic product. The obtained values of LPMO
percentage content in the multienzyme cocktails produced by Penicillium
verruculosum recombinant strains, homologously expressing LPMO, well
correlated with previously calculated data obtained using a delicate
chromatographic fractionation of these preparations followed by estimation of
their qualitative and quantitative content by electrophoresis,
mass-spectrometry and determination of protein concentration in the
chromatographic fractions.
Key words: lytic polysaccharide
monooxygenase, Penicillium verruculosum, cellulose, 2,6-dimetoxyphenol.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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