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A. A. Vartanian, Yu. A. Khochenkova, E. N. Kosobokova, M. A. Baryshnikova, V. S. Kosorukov
CD437 reduces metastatic potential of melanoma
cells
Abstract
Synthetic retinoid CD437, an agonist of the
retinoic acid receptor γ (RARy), in xenograft models
demonstrates high potential for cancer treatment. The aim of this study was to
investigate the melanoma cells metastatic potential alterations induced by
CD437. 2D- and 3D-cell culture, migration “into the wound”, invasion, colony
forming assay and flow cytofluorimetry were used in this study. Here we show
that CD437 reduced the migration of Mel Z melanoma cells by 52 ± 2% compared to
the positive control. The decrease in invasive activity of melanoma cells under
the conditions of CD437 treatment did not exceed 40 ± 4%. CD437 also blocked
the formation of capillary-like structures by melanoma cells on Matrigel. The
efficiency of colony formation by Mel Z cells in the control was quite high.
However, we did not observed any colonies after 7 days of melanoma cells
cultivation with non cytotoxic concentrations of CD437. Further, we showed that
the expression of MMP-9 in Mel Z cells was significantly lower than the
expression of MMP-2. CD437 reduced the expression of MMP-2 by half, and MMP-9
by 35 times compared to the control. We did not reveal the effect of CD437 on
CD44 expression by melanoma cells, there was a slight decrease in CD24
expression (23 ± 2%). The data obtained suggest that CD437 reduces metastatic
potential of melanoma cells.
Key words: CD437, melanoma cells, metastasis, MMP-2,
MMP-9,
CD44,
CD24.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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