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E. E. Kurdyukov, O. A. Vodop’janova, I. Ya. Moiseeva, E. F. Semenova
METHOD OF QUANTITATIVE DETERMINATION OF THE
AMOUNT OF FLAVONOIDS IN THE LEAVES OF MORINGA OLEIFERA
Abstract
The quantitative
determination of the amount of flavonoids in the leaves of Moringa oleifera by
differential spectrophotometry was carried out. Analytical maxima of absorption
of flavonoids of the leaves of Moringa oleifera – 290 nm (shoulder) and 330 nm
(maximum) were determined. It was found that in the presence of aluminum
chloride, moringa flavonoids form a complex compound with
an absorption maximum of 408 nm. Optimal conditions for the extraction of
flavonoids from the raw materials of this plant (extractant-ethyl alcohol 70 %;
the ratio of “raw material-extractant” – 1:200; extraction time – 90 minutes;
the degree of grinding of raw materials is 1.0 mm).
Key words:oilseed moringa, leaves,
flavonoids, spectrophotometry, rutin.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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