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Vasily G. Amelin, Zen
Alabden Chalawi Shogah, Dmitry S. Bolshakov
Microextraction-colorimetric
determination and identification of penicillin antibiotics in medicines using a
smartphone and chemometric analysis
Abstract
A fast and simple
method for the
determination of penicillin antibiotics in medicines is proposed, based on
dispersive liquid-liquid microextraction of penicillin associates with
methylene blue and measuring the colorimetric characteristics of the extracts
using a smartphone and specialized software. As an analytical signal (Ar),
we used the values of the colorimetric parameters in the RGB system. Methods
for determining the content of penicillin G, V, oxacillin, ampicillin,
amoxicillin and nafcillin in drugs have been developed. The limits of detection
and quantitation were 0.2 and 0.6–0.8 µg/ml, respectively. The possibility of
using chemometric analysis, which makes it possible to reduce the time and
visualize research data, is shown. The data set was processed using principal
component analysis (PCA), hierarchical clustering analysis (HCA), and k-means
method using the XLSTAT software. The identification and quantitation of
penicillin antibiotics concentration in tablet forms was carried out using
chemometric analysis. Calibration characteristics are linear with approximation
confidence coefficients ≥ 0.99. The relative standard deviation of the
analysis results does not exceed 0.05. The duration of the analysis was 15–20
min.
Key words:digital
colorimetry, penicillins, dispersive liquid-liquid microextraction, smartphone,
RGB, chemometric analysis
Copyright (C) Chemistry Dept., Moscow State University, 2002
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