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Nadezhda K. Marynich, Alexander P. Savitsky
Definition
of “hotspots” to improve the maturation of the fluorescent protein moxsaasoti
at 37 °C
Abstract
Abstract: In the present work, we searched for amino acid residues, the
replacement of which can contribute to more optimal maturation of the
fluorescent protein moxSAASoti at 37 °C. For many other fluorescent proteins,
an improvement in this characteristic has been obtained by chance through many
rounds of random mutagenesis, however, we were able to find two positions – 74
and 121 – which obviously affect the maturation process of moxSAASoti, which
was verified by introducing substitutions at these positions by the site
directed and site-saturating mutagenesis.
Key words: fluorescent proteins, site-saturating mutagenesis, maturation of
fluorescent proteins
Copyright (C) Chemistry Dept., Moscow State University, 2002
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