Influence of cationic polymers on separation selectivity in kinetic differentiation mode capillary electrophoresis of metal-4-(2-pyridilazo)resorcinolato chelates

O.V. Krokhin, H. Hoshino, O.A. Shpigun, T. Yotsuyanagi

J.Chromatogr. A, v.772(1-2), p.339-346 (1997)

ABSTRACT. The influence of four kinds of cationic polymers on electrophoretic mobilities of V(V), Co(II), Ni(II), and Fe(II) - 4-(2-pyridilazo)resorcinolato chelates was investigated in kinetic differentiation mode capillary electrophoresis (CE). It was shown that the addition of cationic polymers strongly changes selectivity of such a type of separation. Four parameters of cariier electrolyte (pH, counter-ion, polymer and acetonitrile concentration) were changed to examine the elution order of the complexe . The behaviour of the chelates can be described in terms of ion-exchange separation. Fast separation of the four chelates with effeciency of more than 450 000 theoretical plates per meter in CE with reversed polarity power supply was easily performed.

Laboratory of Chromatography