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Serov A. Ye., Tishkov V. I.
Role of the Proline Remains in Stability of Procaryotic and Eucaryotic
Formiate Dehydrogenases
Abstract
Mutagenesis of non-prolyl amino acid residues to proline is one of the
popular methods to stabilize proteins. The influence of these mutations on the
thermal stability of NAD+-specific format dehydrogenases (FDH; KC.
1.2.1.2) from methylotrofic bacterium and baker's yeast was studied. This
stabilizaion method can not be used in the case of the bacterial FDH. Low
stability of FDH from baker's yeast is probably provided by low content of
proline residues in this enzyme. However, the G141P replacement did not
stbilize the yeast FDH. Unlike the bacterial enzyme, FDH from baker's yeast is
possible to be stabilized by increasing rigidity of its polypeptide chain.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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