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Jose L. Martinez H., Iliyna A., Dominguez Malfavon L., Olga Sanchez C., Dustet M. J. C.
Partial characterization of penicillin acylase
from fungi Aspergillus fumigatus and Mucor gryseocianum
Abstract
Preparation of Penicillin G acylase were
obtained using two sources of fungus, Aspergillus
fumigatus H/6.17.3and Mucor griseocyanum H/55.1: The fungi
were propagated in Skim milk medium at 30ºC as the sole nitrient sorce
and D‑Phenylglycine methyl ester as inducer. Dialyzed medium containing
enzyme penicillin G acylase of Mucor
gryseocianum and Aspergillus
fumigatus was used. The phenylmethylsulfonyl fluoride was found to inhibit
Penicillin G acylase activity of obtained preparations. The optimum pH range
for the dialyzed preparations was pH 7-8 and pH 7.5- 8.5 respectively and
optimum temperature for maximal enzyme activity of both sources, was at 40 ºC. Km value determined using
the penicillin G as sustrate of enzymes of Mucor
gryseocianum and Aspergillus
fumigatus was 1.77 x 10-7 M and 1.46 x 10-7 M,
respectively.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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