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A. E. Serov, V. I. Tishkov
Baker’s yeast formate dehydrogenase: unusual mechanism of
inactivation and stabilization by ionic srength and cofactor
Abstract
Mechanism of thermal inactivation of baker’s yeast
formate dehydrogenase (SceFDH) is uniquie for the family of NAD+-dependent
FDHs. The enzyme inactivates in two stages with the first one being reversible,
while the denaturation of other FDHs is described by non-reversible first-order
kinetics. Unusual mechanism of inactivation and low thermal stability of SceFDH
are most probably connected with the flexibility of its polypeptide chain.
SceFDH is observed to be highly stabilyzied by ionic strength and cofactor.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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