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L. Yu. Filatova, Ye. S. Oxenoyt, K. S. Yakovleva, N. I. Ivanova, N. L. Klyachko
Investigation
of Activity and Stability of Papain in Different Micellar Systems
Abstract
Research of hydrolysis
of substrate PyrPheAlaPNa by papain in reverse micelles of Aerosol OT in octane
was made. Optimal conditions of the enzyme functioning in this system were
found. It was established, that in reverse micelles of AOT
in octane dependence of activity of papain on hydration number had two
optimums, which corresponded to activity of monomolecular and oligomeric
papain. As found, addition of nonionic surfactants to Aerosol OT in octane led
to change of catalytic properties of the enzyme. As applied aspect of work
microemulsions consisting of nontoxic.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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