|
V. I. Tishkov, S. V. Khoronenkova, N. V. Cherskova, S. S. Savin, I. V. Uporov
3D-structure
modeling of yeast D-Amino acid oxidase
Abstract
D-amino acid oxidase from yeast Triginiosis variabilis (TvDAAO)
is used in pharmaceutical industry and fine organic synthesis but for future
practical application new mutant forms of the enzyme with improved stability
and catalytic properties have to be prepared. Experiments for crystallization
of TvDAAO were carried out last three decades without any success. For protein
engineering of the enzyme by rational design approach the model 3D-structure of
TvDAAO was built using homology modeling method. Cys108 and Cys298 residues
were prposed for site-directed mutagenesis after analysis of the enzyme model
structure.
Copyright (C) Chemistry Dept., Moscow State University, 2002
|
|
