|
T. V. Sukhanova, I. A. Prudchenko, E. S. Efremov, S. V. Uglanova, L. Yu. Filatova, E. A. Markvicheva, N. L. Klyachko
Biomolecules
in colloid nanocarriers for drug delivery: entrappment and properties of
delta-sleep inducing peptide
Abstract
Nanoemulsions of water in oil type using non-toxic components on the
base of AOT (5%) and soya bean lecithin Lipoid S100 (10%) in eucalyptus oil and
limonene were developed and characterized. As shown, AOT based systems
possessed maximal solubilization capacity (12% of aqueous solution); sizes
measured with photon-correlation spectroscopy were in linear dependence with
water content. As shown, hydrophilic delta-sleep inducing peptide (DSIP)
(regulatory neuropeptide) was able to be included into nanoemulsions of w/o type
in the absence and presence of biopolymeric additives. DSIP entrapment into
nanoemulsion led to its significant stabilization (80–90% after 2 months of
incubation at 22oC in contrast to 28% in water). Peptide release
kinetics was studied in model in vitro experiment (dialysis) and
significant retardation of DSIP release from nanoemulsion in comparison with
water was found. DSIP containing nanoemulsion systems useful for medicine and
cosmetics can serve as a base for development of novel stable drug formulations
of durable action.
Copyright (C) Chemistry Dept., Moscow State University, 2002
|
|
