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M. I. Koksharov, D. V. Smirnova, S. G. Abbasova, N. N. Ugarova
Fusion protein of
luciferase Luciola mingrelica–biotin carboxyl carrier protein:
production, properties, application
Abstract
A plasmid was constructed by genetic
engeneering methods that codes a fusion protein (4TS-bccp87) including the
thermostable mutant of Luciola mingrelica firefly luciferase (4TS) and
the carboxyl-terminal 87 residues of E. coli biotin carrier protein
(bccp87).It was shown that about 60% of the fusion protein was
biotinilated after the expression in E. coli BL21(DE3). Catalytic
properties, thermostability and bioluminescence spectra of the fusion protein
were shown to be similar to that of the initial luciferase. It was demonstrated
that streptavidin-biotinylated luciferase complex can be used in ELISA for the
assay of Salmonella typhimurium cells in the interval from 104 to
5·106 CFU/ml.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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