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V. A. Safronova, J. V. Samsonova, V. G. Grigorenko, A. P. Osipov
Latheral
flow immunoassay of progesterone
Abstract
A new express method based on latheral flow immunoassay (LFIA) for
progesterone detection was developed. To increase the assay sensitivity an
enzyme label (horse-radish peroxidase) was used instead of colloidal gold. An
optimal assay format was chosen and the influence of a range of buffer
supplements (detergents, proteins and sucrose) was investigated by
enzyme-linked immunosorbent assay (ELISA). Linear range of LFIA was between 2
and 40 ng/ml in buffer. Limit of detection was 2 ng/ml, assay time was within
15 min.
Key words: progesterone, latheral flow immunoassay, horseradish peroxidase.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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