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S. G. Dmitrienko, A. V. Stepanova, V. A. Kudrinskaya, V. V. Apyari
Peculiarities
of flavonoids separation by reversed-phase high-performance chromatography on Luna
5u C18(2) column
Abstract
The
chromatographic behavior of several flavonoids (quercetin, naringenin, chrysin,
morin, rutin and naringin) on Luna 5u C18(2) column was studied. Some
peculiarities of retention depending on hydrophobicity of flavonoids were
compared. It was shown that using eluent containing 25 % of acetonitrile and 75
% of 0.1 % H3PO4 water solution made it possible the
separation of rutin, morin, quercetin and naringenin (the time of analysis is
28 min). It was shown that using eluent containing 50 % of acetonitrile made
it possible the separation of quercetin, naringenin and chrysin (the time of
analysis is 8 min) or morin, naringenin and naringin (the time of analysis is 8
min). The method was applied for determination of quercetin in grape polyphenol
food concentrate “Enoant” and extracts from medicinal plants.
Key words: flavonoids, HPLC, Luna 5u C18(2), hypercrossed polystyrene.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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