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A. V. Sklyarenko, O. V. Berezina, D. E. Satarova, V. V. Fedorchuk, E. A. Fedorchuk, S. S. Savin, S. V. Yarotsky, V. I. Tishkov
Recombinant
alpha-amino ester hydrolase from Xanthomonas rubrilineans VKPM B-9915 is
highly efficient biocatalyst of cephalexin synthesis
Abstract
Recombinant
as well as native alpha-amino ester hydrolase from Xanthomonas rubrilineans
VKPM B-9915 (XrAEH, KF 3.1.1.43) was tested for synthesis of
amino-beta-lactamantibiotccephalexin. It was shown that recombinant enzyme r-XrAEH
produced by Escherichia coli VKPM B-11246 is more efficient compared to native one, wt-XrAEH prepared from mutant strain Xanthomonas rubrilineans
VKPM B-9915. Addition of ethylene glycol to reaction
mixture (33 v/v%) results in increase of maximum yield
of cephalosporin from 75 to 95% using as biocatalyst r-XrAEH.
At optimal synthesis conditions change of r-XrAEH
with wt-XrAEH results in decrease of cephalosporin maximum
yield from 95 to 85%. This effect is caused by presence of beta-lactamase side activity in wt-XrAEH preparation.
Key words:
alpha-amino ester hydrolase, recombinant enzyme, cephalexin, synthesis.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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