|
M. A. Orlova, A. A. Poloznikov, A. P. Orlov
Magnetic isotope effect as a tool of
apoptosis modulation in leukemic cells
Abstract
Lymphocytes from healthy donors and
leukemic cells of patients with acute B-lymphoblastic leukemia (BALL-1) and
acute myeloid leukemia was exposed with nanoparticles bearing magnetic (Zn-67)
and nonmagnetic (total isotope pool) nuclei of zinc. The values of
corresponding magnetic isotope effects determined as the ratio of LD50
magnitudes of preparations with magnetic and nonmagnetic zinc isotopes was 0,
3.5 and 1.5. The morphologic studies by confocal and fluorescence microscopy
showed an apoptotic death of cells under preparations and besides there was an
increase in the cell aggregation and better aggregation of nanoparticles in the
case of 67Zn-NP, resulted in a decrease of cytotoxicity. However
magnetic isotope effect was observed even in the case of aggregation.
Key words: porphyrin-fullerene
nanoparticles, aggregation, zinc-67, magnetic isotope effect, B-ALL, AML,
apoptosis, angiotensin-converting enzyme.
Copyright (C) Chemistry Dept., Moscow State University, 2002
|
|
