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T. O. Samarina, M. K. Beklemishev
Determinationof amikacin by rayleigh scattering method based on the covalent bonding of the analyte with a water
soluble polymer
Abstract
A method for
the determination of aminoglycoside antibiotics based on the covalent bonding
of the analyte with a water-soluble polymer by Rayleigh scattering method (λex = λem) is proposed. Determination of amikacin (as model
analyte) is carried out by the carbodiimide crosslinking technique that forms
amide bonds between the co-polymer of 4-styrenesulfonic
acid and maleic acid and primary amine groups of amikacin. Conditions of the reaction have been selected to obtain maximum
scattering intensity of the product: reaction time 15 min, pH 7.0, ionic
strength 0.02, measuring of
scattering intensity at 362 nm. The determination of amikacin is not influenced
by (mol/mol ratio): Al(III), Co(II), Cu(II), Fe(III), Ni(II), Zn(II) – 300, Cl–
and NO3–– 250, Ca(II) and Mg(II) – 50, HCO3–
– 20; nitrogen compounds affect at 20 – 50-fold, protein – 2.5-fold ratio. The
calibration plot was constructed over the range of 0.2– 35 mg/L (r =
0.993), LOD(3s-criterium) is 0.08 mg/L (n = 10, P =
0.95). The analyses on the drug powder for injection and gel have been
performed.
Key words: Rayleigh scattering method,
aminoglycosides, amikacin, carbodiimide cross-linking technique, drug analysis.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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