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Y. A. Denisenko, D. A. Merzlov, A. V. Gusakov, A. V. Chekushina, A. P. Sinitsyn
Comparative
characterization of xylanases XylA AND XylE from the fungus Penicillium
canescens
Abstract
Two xylanases (XylA and XylE), belonging to family 10 of glycoside
hydrolases, were isolated from an enzyme preparation produced by the fungus Penicillium
canescens. Kinetics of glucuronoxylan and arabinoxylan hydrolysis, as well
as the influence of proteinaceous (XIP-like) inhibitors from rye on the XylA
and XylE activity, were studied. XylA provided more complete conversion of
glucuronoxylan than XylE, while XylE was more effective in hydrolysis of
arabinoxylan. Unlike XylA, XylE was resistant to rye proteinaceous inhibitors,
the property rarely met among the family 10 enzymes. Thus, XylE is a
perspective enzyme for application as a cereal feed additive, while XylA may
potentially be used for biobleaching of cellulose from hardwoods, which contain
glucuronoxylan as one of major components.
Key words: xylanase, Penicillium canescens, proteinaceous
inhibitors, xylan, hydrolysis kinetics.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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