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D. A. Matolygina, N. S. Dushutina, E. D. Ovchinnikova, N. L. Eremeev, N. G. Belogurova, D. L. Atroshenko, S. A. Smirnov, S. S. Savin, V. I. Tishkov, A. V. Levashov, P. A. Levashov
Enzymatic lysis
of living microbal cells: universal approach for cell lysis rate calculation in
turbidimetric measurments
Abstract
Turbidimetric
measurerment data for enzymatic lysis of various living bacteria cells are
analyzed. A method for the correct recalculation of turbidimetric data (–ΔA/Δt) into absolute values of living cells concentration change (–ΔCFU/Δt)
is proposed. Dimensionless efficiency of cell lysis –(1/CFU0).ΔCFU/Δt
for various bacteria cells is calculated to correctly compare the efficacy of
different bacteriolytic factors on various bacterial cells.
Key words:
bacteriolytic activity, turbidimetry, lysozyme, interleukin-2, Bacillus
subtilis, Escherichia coli, Lactobacillus plantarum.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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