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N. Yu. Saushkin, J. V. Samsonova, A. P. Osipov, S. E. Kondakov, E. S. Lysova, I. A. Elizarova, K. S. Khaertynov, E. A. Shuralev
Strip-dried
biofluids for the detection of specific antibodies in infected small ruminants
Abstract
Comparative
determination of specific antibodies in goats for viral arthritis-encephalitis
and toxoplasmosis by ELISA and latex agglutination reaction using strip-dried
serum and whole blood samples obtained on a porous membrane carrier was
performed. It was shown that the use of strip-dried samples allows qualitative
and quantitative determination of specific antibodies at a level completely
concurred with that of liquid samples (serum). This method of sample
preparation and analysis can be used for safe blood samples shipment and
following serological studies for epizootic monitoring.
Key words:
caprine arthritis encephalitis, toxoplasmosis, ELISA, latex agglutination test,
strip-dried biofluids, dbs (dry blood spots).
Copyright (C) Chemistry Dept., Moscow State University, 2002
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