V. A. Kryachkov, V. N. Tashlitsky, A. V. Bacheva
Design and
characteristics on novel proteasome substrates containing oligoQ sequence
Abstract
Fluorogenic
polyglutamyl-containing peptides with 5 or 10 Gln residues and FRET-pair EDANS
(fluorophore) and Dabcyl (quencher) were characterized by spectral and
mass-spectrometry techniques. The possibility for 20S proteasome to hydrolyze
these modified peptides was examined. Hydrolysis kinetics was studied and
catalytic efficiency for the substrates was determined. It was shown that
presence of glycine dramatically decreased the solubility of the substrate and
also diminished the efficiency of the hydrolysis.
Key words: proteasome, fluorogenic substrates, proteasome activity, polyglutamine
diseases.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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