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V. G. Amelin, D. S. Bol’shakov
Simple preparation, identification and
determination of twelve macrolids with a standard additive in food raw material
and foodstuffs by mass-spectrometry of high-resolution
Abstract
A simple method for extraction,
identification and determination 12 antibiotics-macrolides (azithromycin,
roxithromycin, jozamycin, spiramycin I–III, tilmicosin, tylosin, erythromycin,
clarithromycin, tilowozine and tulatromycin) in food raw materials and
foodstuffs using high-resolution time-of-flight mass spectrometry is proposed.
The ranges of the macrolide content determined were 1–400 ng/g, detection
limits were 0,01–0,5 ng/g, determination limits were 0,04–2 ng/g. A scheme for
the identification and detection of detectable antibiotics by the standard
addition method is proposed. The relative standard deviation of the analysis
results does not exceed 21%. Duration of screening of samples 40 min, the
determination time of detected antibiotics is 1–1,5 h.
Key words: antibiotics-macrolides,
foodstuffs, high-performance liquid chromatography, high-resolution
time-of-flight mass spectrometry.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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