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O. S. Savinova, I. N. Zorov, D. V. Vasina, A. P. Sinitsyn, T. V. Fedorova
The minor recombinant laccase isozymes of Trametes
hirsuta 072: obtaining and properties
Abstract
Laccases (EC 1.10.3.2) are multicopper
polyphenol oxidases found in various organisms, in particular, in fungi. Fungal
laccases are encoded by multigenic families, which can contain up to 17 genes.
However, not all isozymes can be obtained from native producers. Previous
studies have shown that the filamentous fungus Penicillium canescens is
a promising object for the heterologous expression of various laccase isozymes
of Trametes hirsuta 072. In this work, the cultivation conditions of P.
canescens strains, recombinant producers of T. hirsuta heterologous
minor laccase, have been optimized. Optimization of rLacD and rLacF
purification method increased their yield and specific activity of rLacD. In
addition, the melting points of minor laccase isoenzymes were measured and the
glycosylation of isoenzymes was studied.
Key words: laccase, isozymes,Trametes
hirsuta, Penicillium canescens, properties.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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