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D. V. Barkhova, M. S. Andrianova, N. V. Komarova, A. E. Kuznetsov
Channel and motor proteins for translocation of
nucleic acids in nanopore sequencing technology
Abstract
In the process
of nanopore sequencing, the primary structure of the analyzed DNA or RNA is
decrypted by translocation of a nucleic acid molecule through a nanopore
intercalated into the membrane. A nanopore is a protein transmembrane channel
that is permeable to water, low molecular weight ions, and single-stranded DNA
or RNA. This review discusses the channel proteins used in nanopore sequencing,
their modifications, as well as assisting proteins that regulate the process of
nucleic acid translocation.
Key words: cëoâa: nanopore sequencing, organic nanopore, transmembrane protein,
motor protein.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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