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T. A. Bogush, A. A. Basharina, O. S. Burova, E. A. Bogush, V. Yu. Kirsanov, A. M. Scherbakov, V. A. Syuvatkin, O. M. Ryabinina, M. A. Baryshnikova, V. S. Kosorukov
Comparative
immunofluorescent analysis of estrogen receptor beta expression in ovarian and
breast cancer cell lines
Abstract
Comparative
quantitative evaluation of estrogen receptors beta (ERβ) expression was performed by
immunofluorescence method using flow cytometry in ovarian cancer cell lines
COLO-704, OVCAR-3, EFO-21 and SK-OV-3 as well as breast cancer cell line MCF-7.
High level of ERβ
expression (88%–91%) was revealed in all cell cultures investigated, and
significant differences were observed in marker expression intensity between
ovarian cancer cell cultures (in OVCAR-3, EFO-21 and SK-OV-3 are higher
compared with COLO-704 cells in 1,6; 2,3 and 3,5 times, respectively).
Moreover, the intensity of ERβ expression was significantly higher in SK-OV-3, EFO-21 and OVCAR-3 than
in MCF-7 cells: in 2,7; 1,4 and 1,9 times, respectively. The authors suggest
that in contrast to breast cancer patients, ERβ and not ERα can be the main target for antiestrogens in
ovarian cancer patients. The revealed differences in ERβ expression in a number of widely
used ovarian cancer cell cultures let us recommend them for further fundamental
researches of ERβ
to study the biological effects of antiestrogens.
Key words:
estrogen receptors beta, flow cytometry, COLO-704, OVCAR-3, EFO-21, SK-OV-3,
MCF-7.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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