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A. A. Savina, O. A. Voronina, N. V. Bogolyubova, S. Yu. Zaitsev
Amperometric detection of antioxidant activity
of model and biological liquids
Abstract
Active forms of oxygen formed during many metabolic processes are able
to oxidize biologically active compounds. The aim of the work was to study the
total amount of water-soluble antioxidants (TAWSA) in model and biological
liquids by the amperometric method (using the “TsvetYauza 01-AA” instrument).
TAWSA for biologically active ascorbic acid was 46.53 mg/g, for sea buckthorn
0.54 mg/g, for white grapes 0.28 mg/g, for amino acids – about 4 mg/g (average
value at their content in the blood), for cattle blood serum from various sex
and age animal groups (at comparable experimental conditions) from 14.8 mg/g to
21.3 mg/g. The level of TAWSA for young cattle (age 3 months) was significantly
higher than those for lactating animals, which may be due to a higher content
of aromatic amino acids (with high antioxidant effects) in the blood serum of
young individuals. The data obtained are important in assessing the
physiological-biochemical status and state of the body antioxidant defense system.
Key words: analytical
biochemistry, amperometric detection, antioxidant protection, amino
acids, blood serum.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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