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A. A. Kalinina, S. S. Sychev, N. V. Gursky, T. N. Sokolova
Inhibition of intracellular succinate
dehydrogenase of bacteria Bacillus Subtilis as a criterion for the
participation of an enzyme in the reduction of chloride iodonitrotetrazolium -
indicator of cell viability
Abstract
It is shown in the work that after
diffusion of tetrazolium salt to membrane reducing agents of cells of bacteria Bacillus
subtilis, the state of the enzyme succinate dehydrogenase affects the rate
of recovery. Based on the dependence of the initial rate of reduction of
iodonitrotetrazolium chloride by the cellular components of Bacillus
subtilis on the concentration of malonic acid, the constant of competitive
inhibition of the intracellular enzyme succinate dehydrogenase was
quantitatively determined.
Key words: bacteria, iodonitrotetrazolium
chloride, kinetics, inhibition, malonic acid, succinate dehydrogenase enzyme.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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