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L. V. Sigolaeva, A. Yu. Konyakhina, D. V. Pergushov, I. N. Kurochkin
Electrochemical biosensor based on
nanosized polymer-enzyme films composed of linear poly(n,n-dimethylaminoethyl
methacrylate) and choline oxidase
Abstract
Nanosized polymer-enzyme films were
fabricated on graphite surfaces via a sequential two-stage adsorption of the
pH- and thermoresponsive polymer, viz., linear poly(N,N-dimethylaminoethyl
methacrylate) (PDMAEMA), and the enzyme, viz., choline oxidase (ChO). The
influence of the conditions (pH and temperature), which are applied for
deposition of the polymer onto graphite surface, on electrochemical responses
of the polymer-enzyme sensor constructs and kinetic parameters of enzymatic
oxidation of choline by ChO integrated into the polymer-enzyme films was
revealed. Stimuli-sensitivity of PDMAEMA was found to control properties of the
formed polymer coatings (surface coverage, film thickness and roughness), which
decisively determine the amount of the ChO bound by the polymer film and,
consequently, the resultant electrochemical characteristics of the
polymer-enzyme sensor constructs.
Key words: stimuli-sensitivity;
thermoresponsive polymer; polyelectrolyte; surface modification; adsorption;
thin films; biosensor; poly(N,N-dimethylaminoethyl methacrylate);
choline oxidase; electrochemistry; atomic force microscopy.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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