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Varvara D. Makarova, Eduard K. Pisarev, Svetlana A. Pavlova, Galina V. Pavlova, Maria I. Zvereva
Universal
system for determining mutations in the promoter of the TERT gene on the
example of the analysis of tumor lesions of the central nervous system
Abstract
Abstract. In this work, we
compared real-time PCR and droplet digital PCR methods for the promoter region
of TERT gene mutations detection using various systems of detecting
probes. The detection limits of mutations for these methods were determined,
based on which the advantages of the droplet digital PCR method for future
studies of tumor lesions of the central nervous system were described. For the
first time, the mutant allele fraction of changes in the promoter of TERT
gene for cell lines of tumor lesions of the central nervous system was analyzed
as 60% for glioblastoma Grade IV IDH1. To compare these methods, a
versatile system based on recombinant plasmids was proposed, which proved to be
better than the system based on genomic DNA.
Key words: TERT promoter mutations, droplet digital PCR, telomerase reverse
transcriptase, cancer biomarkers
Copyright (C) Chemistry Dept., Moscow State University, 2002
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