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Nikolay V. Rastriga, Daria A. Gasanova, Pavel A. Levashov
Adsorption
of lysozyme on living cells of Escherichia coli and its bacteriolytic
activity in the presence of glycine and charged amino acids
Abstract
Abstract. For human and chicken lysozyme , the relationship between changes in
the parameters of enzyme adsorption on living Escherichia coli bacterial
cells and the value of its effective bacteriolytic activity in the presence of
glycine and charged amino acids was studied . It has been shown for both human
and chicken lysozyme that free amino acids added to a concentration of 1.5 mM
for glycine or 5.0 mM for glutamate, aspartate, histidine, arginine, and lysine
reduce the desorption constant of the enzyme on bacterial cells to 1.4–2.0
times. At the same time, an increase in the bacteriolytic activity of lysozyme
is also observed in 1.5–1.9 times. Thus, the enhancement of
antibacterial activity in the presence of glycine and charged amino acids can
be explained by an improvement in the productive sorption of the enzyme on the
substrate, bacterial cells.
Key words: adsorption of lysozyme, bacteriolytic activity, human lysozyme,
chicken lysozyme, lysozyme activators, Escherichia coli
Copyright (C) Chemistry Dept., Moscow State University, 2002
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