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Anna S. Cherkashina, Olga O. Mikheeva, Vasiliy G. Akimkin
Protein
engineering of bst polymerase for isothermal amplification purposes
Abstract
Abstract. The review is devoted to protein engineering of Bst polymerase using
various methods. To modify the enzyme, approaches such as the production of
chimeric proteins, directed evolution, directed and random mutagenesis have
been used. Examples of successful changes in enzyme properties such as
catalytic activity, processivity, thermal stability, and resistance to
inhibitors are described.
Key words: Bst-polymerase, big fragment, strand displacement activity, isothermal
amplification, mutagenesis
Copyright (C) Chemistry Dept., Moscow State University, 2002
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