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Tatiana A. Bogush, Anna A. Basharina, Ilia P. Romanov, Anna N. Grishanina, Elena A. Bogush, Alexander M. Scherbakov, Anna B. Ravcheeva, Alexey
Lee, Vyacheslav S. Kosorukov
Immunofluorescence analysis of estrogen receptors alpha
expression in non-small cell lung cancer tissue
Abstract
Abstract. Quantitative assessment of estrogen receptors alpha
(ERα) expression was carried out in 115 samples of
non-small cell lung cancer (NSCLC) by an immunofluorescent assay and flow
cytometry. It has been shown that high level of ERα≥20% predicts higher aggressiveness of NSCLC than at low level
<20%: median survival at 78 mos of follow-up is increased by 1.5 times; the
risk of death is reduced by almost 2.0 times (p = 0.04). Time to death
was increased by an average of 18 mos in about 20% of patients with low ERα
expression. The results validate informative value of immunofluorescence
analysis and flowcytometry for quantifying the ERα expression and
substantiate the prospects of antiestrogen therapy as a new option for NSCLC
treatment, in particular, by analogy with breast cancer – in a long-term
adjuvant therapy in ERα+ NSCLC patients.
Key words: non-small cell lung cancer, prognosis, estrogen
receptor alpha, Kaplan-Meier method, immunofluorescence analysis, flow
cytometry
Copyright (C) Chemistry Dept., Moscow State University, 2002
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