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Ludmila A. Frank, Vasilisa V. Krasitskaya
Coelenterazine-dependent
luciferases: properties and application
Abstract
Abstract. The review presents data on the structure of
bioluminescent systems of marine animals that use coelenterazine-dependent
luciferases as light-emitting proteins.It has been shown
that due to the availability, stability and high quantum yield of reactions,
these luciferases, as well as their genetic variants with new useful
properties, are successfully applied as reporter molecules in a variety of
analytical systems in vitro and in vivo.Their use provides high sensitivity, simple design and fast
analysis.
Key words: bioluminescence, coelenterazine, luciferase, Ca2+-regulated
photoprotein, analytical systems
Copyright (C) Chemistry Dept., Moscow State University, 2002
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