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Georgy V. Nesterov, Tatiana M. Litvinova, Vladimir N. Matveenko, Nadezhda V. Nesterova, Fidan Sh. Suleymanova
Identification
and quantification of triterpene saponins in the infructescence and leaves of
various alder species
Abstract
Abstract. The presence of triterpene saponins was established using
qualitative reactions in the seed and leaves of gray alder (Alnusincana (L.)
Moech) and black (sticky) (Alnus glutinosa (L.) Gaerth). The presence of
oleanolic acid in all studied samples was established by thin layer chromatography.
Evaluation of the quantitative content of the sum of triterpene saponins in
terms of oleanolic acid was carried out by direct spectrophotometry using a
Specord instrument at a wavelength of 310 nm. It has been shown that the
content of the sum of triterpene saponins in terms of oleanolic acid is from
1.538 to 1.682% for infructescence, and from 1.102 to 1.274% for leaves.
Key words: black alder, gray alder, alder leaves, alder infructescence,
oleanolic acid, triterpene saponins, thin layer chromatography,
spectrophotometry
Copyright (C) Chemistry Dept., Moscow State University, 2002
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