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Makeeva A. A., Shumilkin A. S., Ryzhkova A. S., Vernaya
O. I., Shabatin A. V., Semenov A. M., Shabatina T. I.
Biopolymer
controlled release systems based on hydrolyzed collagen: relationship of
cryoforming conditions, structure and properties
Abstract
Abstract. Biopolymer materials based on natural collagen (gelatin and
hydrolyzed collagen) are widely used in the food, pharmaceutical and cosmetic
industries due to their low toxicity, high biocompatibility, low antigenicity,
and unique mechanical and technological properties. Hydrolyzed collagen, unlike
gelatin, is formed by peptides with a lower molecular weight. It has higher
bioavailability and biodegradability compared to gelatin. In this work, using
low-temperature technologies, biopolymer matrices based on hydrolyzed collagen
containing the antibacterial drug dioxidine were obtained. It has been shown
that by varying such synthesis parameters such as the concentration of
hydrolyzed collagen in the precursor solution (from 1 to 10%), matrix
cross-linking time (0.1–24 hours), cryoforming temperature (–30 and –196 °C) it
is possible to change the morphology and structure matrix, its degradation time
and drug release time. The composition and structure of dioxidine/hydrolyzed
collagen systems were characterized by SEM, IR and UV spectroscopy. The
antibacterial activity of the resulting dioxidine/hydrolyzed collagen systems
against E. coli and S. aureus was characterized by the disk
diffusion method.
Key words: hydrolyzed collagen, dioxidine, cryomodification, low temperatures,
controlled release, medical biopolymer materials
Copyright (C) Chemistry Dept., Moscow State University, 2002
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