Samsonova Zh. V., Rubtsova M. Yu., Chikisheva L. V., Yegorov A. M.
Membrane Immune Analysis of Chloramphenicol
Abstract
Bioluminescent assay of total bacterial contamination (total number of
mesophilic aerobic and facultative anaerobic microorganisms) of raw processed
meat, based on determination of intracellular bacterial ATP was developed. For
elimination of non-bacterial ATP the sample was treated with mixture of detergent and protease
followed by filtration through bacterial membrane filter. For simplification of
the assay the special luminometric microcuvette FiltravetteTM was
used. The bottom of FiltravetteTM is
made of bacterial membrane filter. This design allows to concentrate bacteria
cells, release intracellular ATP and measure bioluminescent signal in the same
cuvette. Combined application of the highly sensitive ATP-reagent based on
recombinant firefly luciferase which was developed in our laboratory, and
FiltravetteTM allows to detect bacterial contamination with
detection limit 103 CFU per/g meat. The duration of the assay is
about 100 min while the standard Plate Count takes 48 h. A good correlation
between bioluminescent assay and the standard Plate Count was observed
(R=0,99).
Copyright (C) Chemistry Dept., Moscow State University, 2002
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