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Zhmaeva Ye. V., Bychkov P. V., Shekhovtsova T. N.
Determination of Microquantities of Hg(II) and Methylmercury Using
Alcohol Dehydrogenases of Different Origin
Abstract
It was shown that Hg(II) and methylmercury are the most effective
inhibitors (mixed and un-competitive types, respectively) of alcohol
dehydrogenases isolated from baker’s yeast and equine liver. Here, first enzyme
mentioned is more sensitive to the action of these toxicants. Applying the
reaction of ethanol oxidation with nicotineamideadenine dinucleotide catalyzed
by alcohol dehydrogenases from baker’s yeast and equine liver, the procedures
for the determination of mercury and methylmercury were developed with CL=0.25,
10 nM and 10 nM, 1 μM (sr=0.02-0.05).
Copyright (C) Chemistry Dept., Moscow State University, 2002
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