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O. A. Kost, M. N. Petrov, I. A. Naperova, V. A. Tikhomirova, O. V. Kryukova, I. V. Gachok, N. I. Bulaeva, E. Z. Golukhova, S. M. Danilov
Conformational
"fingerprinting" of angiotensin-converting enzyme in the blood in health and
disease
Abstract
The pattern of binding of the set of 16 monoclonal antibodies to the
different epitops on the surface of two domains of angiotensin-converting
enzyme – “conformational fingerprinting” – allows to reveal in the blood the
presence of the enzyme, which is not produced by lung endothelial cells, but by
other cells, e.g. by Gaucher’s cells and sarcoid granuloma cells. We showed the
existence of conformationally changed angiotensin-converting enzyme in the
blood of patients with uremia, this enzyme being characterized by the enhanced
activity towards angiotensin I and decreased ability to be inhibited by
specific inhibitors. The perspectives of the discovering of conformationally
changed enzyme in the blood at atrial fibrillation are also discussed.
Key words:
angiotensin-converting enzyme, monoclonal antibodies, conformation, tissue
specificity.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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