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N. Yu. Saushkin, J. V. Samsonova, A. P. Osipov, C. E. Kondakov, N. I. Khammadov, K. V. Usoltsev, Kh. Z. Makaev, A. N. Chernov
Comparison of PCR and ELISA methods for
detection of bovine leucosis in dried blood spots
Abstract
PCR and ELISA methods for detection of
bovine leucosis in dried blood spots on porous membranes were compared. Dried
samples were analyzed by real-time PCR using several diagnostic test-systems.
19 and 20 samples were detected as positive by PCR and ELISA, correspondently.
14 samples among them were positive in both methods. PCR and ELISA recovered 26
positive samples (47%) in total. The results of analysis of dried and native
samples were in good agreement. Obtained results showed that whole blood
sampling in a form of dried spots on membrane can be used as a convenient and
reliable way for
biological fluid sampling for bovine leucosis screening.
Key words: enzootic bovine leucosis, ELISA,
PCR, porous membranes, dried blood spots.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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