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A. A. Pometun, N. S. Voinova, E. V. Pometun, S. S. Savin, V. I. Tishkov
Impact
of medium pH and ion strength on thermal stability of plant formate
dehydrogenases
Abstract
Impact of medium pH and ion strength on thermal
stability of formate dehydrogenases (EC 1.2.1.2, FDH) from model plant Arabidopsis
thaliana (AthFDH) and soya Glycine max (SoyFDH) have been studied.
Dependence of residual activity on time followed kinetics of reaction of the
first order at all experimental conditions.. Dependences of first order thermal
inactivation constants showed bell-shape curve. It was found that pH increase
resulted in growth of inactivation rate constant for SoyFDH and decrease of one
for AthFDH. Activation parameters of thermal inactivation process, ΔH≠ and
ΔS≠, have been calculated from experimental data
at different medium pH values and phosphate buffer concentrations.
Key words: formate dehydrogenase, thermal stability, impact of medium
parameters, activation parameters of thermal inactivation process.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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