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T. A. Bogush, A. A. Basharina, E. A. Bogush, A. N. Grishanina, D. M. Sakaeva1, V. Yu. Kirsanov, M. M. Davydov, V. S. Kosorukov
Immunofluorescent
assay of de novo vimentin expression in ovarian cancer tissues: surgical
specimens vs paraffin-embedded tissue blocks
Abstract
The results
of analysis of epithelial-mesenchymal transition in serous ovarian cancer were
compared by the developed method of double immunofluorescent staining and flow
cytometry of different tumor material types (surgical specimens vs
paraffin-embedded tissue blocks). Estimation of de novo coexpression of
mesenchymal protein vimentin in epithelial tumor cells expressing cytokeratins
showed that the results were similar not only in quantitative terms, but also
visually in the way of cell distribution on flow cytometry dot plots. The
obtained results indicate prospects of the developed new technology for
molecular diagnostics of tumor epithelial-mesenchymal transition into routine
laboratory practice.
Key words: immunofluorescent
assay, flow cytometry, vimentin, cytokeratins, paraffin-embedded tissue blocks,
epithelial-mesenchymal
transition.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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