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T. A. Bogush, E. M. Kapura-Brekhovskikh, A. A. Basharina, E. A. Bogush, V. Yu. Kirsanov, O. B. Abu-Khaidar, P. A. Lozbina, N. O. Vikhlyantseva, V. S. Kosorukov
Comparative
immunofluorescence analysis of beta-III tubulin (TUBB3) expression in gastric
cancer tissue and morphologically normal tissue adjaced to the tumor
Abstract
Immunofluorescence analysis of
tumor-associated protein TUBB3 in gastric cancer tissue and morphologically
normal tissue adjacent to the tumor was carried out using flow cytometry (in
total – 48 samples). Expression of TUBB3 was detected in 100% of the tumors and
in 79.2% of the “normal” tissue samples. In 36.8% of “normal” tissue samples
the marker expression level was approximately equal to that in the tumor. It
was significantly (1.8-fold) lower in 63.2% of the cases. Direct correlation
was found between the level of TUBB3 expression in tumor and “normal” tissue (p
= 0.0061). It is concluded that the TUBB3 expression in morphologically normal
tissue adjacent to the tumor could be a molecular marker of the local spread of
gastric cancer and a guideline for the optimal adjuvant treatment.
Key words: beta-III tubulin (TUBB3), flow
cytometry, gastric cancer, immunofluorescence staining, morphologically normal
tissue adjacent to the tumor.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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