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T. A. Bogush, M. A. Maiak, N. S. Saprykina, A. A. Basharina, O. M. Ryabinina, A. N. Grishanina, E. A. Bogush, A. A. Kamensky, V. S. Kosorukov
Experimental verification of the hypothesis on
the Lewis lung carcinoma model about the
possibility of molecular diagnostics of local tumor spread
Abstract
Expression of tumor-associated protein beta-III
tubulin (TUBB3) was evaluated quantitively by immunofluorescence analysis using
flow cytometry in lung tissue of intact animals and on day 11 after the Lewis
lung carcinoma transplantation as well in lung metastasis tissue and in
visually “normal” surrounding tissue. It was shown that the tumor is
characterized by a high TUBB3 expression, while also the expression of the
marker was detected in both variants of visually “normal” lung tissue: it was
lower than in tumor tissue but significantly higher than in lung tissue of
intact animals. The detection of TUBB3 outside the tumor indicates that the
tissue appeared to be normal is already involved in malignancy and this
supports the hypothesis that tumor-associated protein TUBB3 can be used as a
molecular marker of local tumor spread.
Key words: beta-III tubulin (TUBB3), flow
cytometry, immunofluorescent analysis, lung cancer, tissue surrounding tumor.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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