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Vladimir I. Tishkov, Anastasia A. Pometun, Svyatoslav S. Savin
Formate
dehydrogenase: from NAD(P)h regeneration to the target in pathogens biofilms, a
component of highly efficient hybrid biocatalysts and CO2 fixation
from the atmosphere
Abstract
Abstract. NAD(P)+-dependent
formate dehydrogenase (EC 1.2.1.2, FDH) catalyzes the simple from chemical and
biological point of view reaction of formate ion oxidation to carbon dioxide
with corresponding reduction of NAD(P)+ to NAD(P)H. Advances in the
life sciences have shown that this reaction plays an extremely important role
in a wide variety of organisms. The areas and types of practical applications
of FDH are also permanently expanding. In this review we considered the main
stages in the development of understanding and knowledge about the role of
formate dehydrogenase in living systems. Achievements in creation of highly
efficient catalysts based on FDH for classic biotechnology as well as for new
areas are also considered. The importance of appropriate choice of the initial
FDH for the creation of a biocatalyst with the required and prescribed
properties with minimal costs is shown. The prospects for the use of FDH for
the fixation of CO2 are discussed.
Key words: formate dehydrogenase, main properties, catalytic
mechanism, physiological role, practical applications, protein engineering,
hybrid biocatalysts, CO2 fixation
Copyright (C) Chemistry Dept., Moscow State University, 2002
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