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Andrey Vladimirovich
Pirogov, Sergey Mikhailovich Staroverov
Simultaneous
determination of chlorhexidine, p-chloraniline, triclosan, dibutyl
phthalate, and ionol in antibacterial liquid by hplc with spectrophotometric
detection
Abstract
Adstract. The article is devoted
to the development of a simple chromatographic method for the simultaneous
determination of the well-known antiseptics – chlorhexidine and triclosan – in
new generation of antiseptic liquids. The dependences of the retention times of
chlorhexidine and triclosan on the acetonitrile concentration in the mobile
phase (from 30 to 60%) and pH (in the range of 3–8 units) for sorbents Luna
C18, Nucleosil CN, Diaspher C4 were studied. A perfect separation of
chlorhexidine, p-chloraniline (the most toxic product of chlorhexidine
decomposition), dibutylphthalate (as a component of cosmetic alcohol),
triclosan and ionol (butylhydroxytoluene, BHT, E321 – antioxidant agent) during
20 minutes in the gradient elution mode on the column with a domestic sorbent
Diaspher C4 has been demonstrated.
Key words: high performance liquid chromatography,
chlorhexidine, triclosan, antiseptic liquids
Copyright (C) Chemistry Dept., Moscow State University, 2002
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