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Alexey
A. Titov1, Alexander E. Kuznetsov2, Natalia V. Komarova1
Optimization
of covalent DNA immobilization for contact printing method
Abstract
Abstract. The contact printing method is widely used to create
microchips and develop highly sensitive biosensors. This method is based on
techniques for immobilizing biomolecules. In this work, two effective methods
for covalent immobilization of oligonucleotides, based on the reactions of
disulfide bond formation and azide-alkyne cycloaddition, were optimized for the
contact printing method. With both immobilization strategies, oligonucleotides
retain the ability to hybridize with complementary DNA, which provides the
basis for their practical use in bioanalytical applications.
Key words: immobilization, DNA, microchips, contact printing
Copyright (C) Chemistry Dept., Moscow State University, 2002
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