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K. U. Fedorchenko, A. M. Ryabokon, A. S. Kononikhin, S. I. Mitrofanov V. V. Barmin, O. V. Pikin, E. H. Anaev, I. V. Gachok, I. A. Popov, E. N. Nikolaev, A. G. Chuchalin, S. D. Varfolomeev
Lung cancer early diagnosis based on
proteome analysis of exhaled breath condensate
Abstract
Using the method of ion cyclotron resonance
mass spectrometry with electrospray ionization we conducted a comparative study
of exhaled breath condensate (EBC) proteome obtained for four donor groups:
diagnosed with lung cancer, diagnosed with chronic obstructive pulmonary
disease, diagnosed with community-acquired pneumonia and healthy nonsmoking
control. More than 300 proteins were identified; 19 proteins were allocated for
the EBC samples of donors diagnosed with the early stages of lung cancer as
potentially significant in the development of diagnostic panel of lung cancer
biomarkers. We have shown the protein profiles of different donor groups to be
subjected to separation and selection of a particular group of proteins for
certain conditions/diseases of the respiratory system. Thus the EBC analysis
could be a promising non-invasive method for lung cancer early diagnosis.
Key words: lung cancer, early diagnosis,
exhaled breath condensate, proteomic analysis, HPLC-MS/MS.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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